Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells

被引:0
|
作者
Seki, Takahiro [1 ]
Mineshima, Ryota [1 ]
Hashimoto, Michihiro [1 ]
Matsumoto, Kouji [1 ]
Hara, Hiroshi [1 ]
Matsuoka, Satoshi [1 ]
机构
[1] Saitama Univ, Grad Sch Sci & Engn, Dept Biochem & Mol Biol, Sakura Ku, Saitama, Saitama 3388570, Japan
基金
日本学术振兴会;
关键词
anti-sigma factor; Bacillus subtilis; ECF sigma factor; Escherichia coli; glucolipid; LIPOTEICHOIC ACID SYNTHESIS; GROWTH; CARDIOLIPIN; EXPRESSION; MUTANTS; PROTEIN;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Extracytoplasmic function (ECF) sigma factors respond to environmental stresses and regulate numerous genes required for adaptation. Under normal growth conditions, the ECF sigma factors are sequestered by transmembrane anti-sigma factor proteins, from which they are released under stress conditions. In Bacillus subtilis ugtP null mutant cells, which lack glucolipids, three of the seven ECF sigma factors, sigma(M), sigma(V) and sigma(X), are activated. The Escherichia coli cell membrane does not contain glucolipids. When the genes for these three ECF sigma and anti-sigma factors were introduced into E. coli cells, expression of lacZ fused to the ECF sigma factor-regulated promoters indicated ECF sigma factor activity. Additional expression of the ugtP gene in these E. coli cells led to the synthesis of small amounts of glucolipids, and the activities of sigma(M) and sigma(V) were repressed, but the activity of sigma(X) was unaffected. It is likely that glucolipids directly influence anti-sigma(M) and anti-sigma(V) factors by stabilizing conformations that sequester the respective ECF sigma factors.
引用
收藏
页码:109 / 114
页数:6
相关论文
共 50 条
  • [31] Extracytoplasmic Function σ Factors Regulate Expression of the Bacillus subtilis yabE Gene via a cis-Acting Antisense RNA
    Eiamphungporn, Warawan
    Helmann, John D.
    JOURNAL OF BACTERIOLOGY, 2009, 191 (03) : 1101 - 1105
  • [32] SECRETION ACTIVITIES OF BACILLUS-SUBTILIS ALPHA-AMYLASE SIGNAL PEPTIDES OF DIFFERENT LENGTHS IN ESCHERICHIA-COLI-CELLS
    NAKAZAWA, K
    TAKANO, T
    SOHMA, A
    YAMANE, K
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 134 (02) : 624 - 631
  • [33] EXPRESSION OF GENES OF BACILLUS-SUBTILIS LYSIN BIOSYNTHESIS IN ESCHERICHIA-COLI-CELLS
    SHEVCHENKO, TN
    OKUNEV, OV
    ALEKSIEVA, ZM
    MALYUTA, SS
    TSITOLOGIYA I GENETIKA, 1984, 18 (01): : 58 - &
  • [34] Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution
    Seaver, M
    Roselle, DC
    Pinto, JF
    Eversole, JD
    APPLIED OPTICS, 1998, 37 (22): : 5344 - 5347
  • [35] Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors
    Yoshimura, M
    Asai, K
    Sadaie, Y
    Yoshikawa, H
    MICROBIOLOGY-SGM, 2004, 150 : 591 - 599
  • [36] Two extracytoplasmic function sigma subunits, σE and σFecI of Escherichia coli:: Promoter selectivity and intracellular levels
    Maeda, H
    Jishage, M
    Nomura, T
    Fujita, N
    Ishihama, A
    JOURNAL OF BACTERIOLOGY, 2000, 182 (04) : 1181 - 1184
  • [37] Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ
    Sorokin, A
    Bolotin, A
    Purnelle, B
    Hilbert, H
    Lauber, J
    Dusterhoft, A
    Ehrlich, SD
    MICROBIOLOGY-SGM, 1997, 143 : 2939 - 2943
  • [38] New Antimicrobial Peptide with Two CRAC Motifs: Activity against Escherichia coli and Bacillus subtilis
    Koksharova, Olga
    Safronova, Nina
    Dunina-Barkovskaya, Antonina
    MICROORGANISMS, 2022, 10 (08)
  • [39] Comparative Secretory Efficiency of Two Chitosanase Signal Peptides from Bacillus subtilis in Escherichia coli
    Eom, Tae-Yang
    Gang, Yehui
    Lee, Youngdeuk
    Kang, Yoon-Hyeok
    Jo, Eunyoung
    Marasinghe, Svini Dileepa
    Park, Heung Sik
    Park, Gun-Hoo
    Oh, Chulhong
    JOURNAL OF MICROBIOLOGY, 2024, : 1155 - 1164
  • [40] Two Types of Transcriptional Repression in Living Cells of Bacillus Subtilis Characterized by Number and Brightness Analysis
    Ferguson, Matthew L.
    Le Coq, Dominique
    Jules, Matthieu
    Chun, Bryan
    Aymerich, Stephane
    Radulescu, Ovidiu
    Declerck, Nathalie
    Royer, Catherine A.
    BIOPHYSICAL JOURNAL, 2011, 100 (03) : 175 - 176