Analysis of Polyphenols in Apple Pomace using Gas Chromatography-Mass Spectrometry with Derivatization

N, O-double (three methyl silane) trifluoro ethyl amide was used to derivatize polyphenols in apple pomace, and the resultant products were tested. The optimal derivatization conditions were then used in conjunction with the rapid analysis of polyphenols by gas chromatography-mass spectrometry. In the results, the following conditions for derivatization with N, O-double (three methyl silane) trifluoro ethyl amide were obtained : Polyphenols should be derivatized for 12 h at 37.5(o)C with a mass ratio of derivatization reagent to sample being 10 to 1. After analysis through gas chromatography-mass spectrometry, 24 compounds were identified, 11 of which were phenolic compounds and organic acids. Furthermore, four isomeric compounds of epicatechin were separated out. The method proved to be easy to use and sensitive, and could be used to analyze unknown phenolic compounds.