PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains

被引：12
|
作者：
Steinhauserova, I [1 ]
Ceskova, J [1 ]
Nebola, M [1 ]
机构：
[1] Univ Vet & Pharmceut Sci, Dept Meat Hyg & Technol, Brno 61242, Czech Republic
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2002年 / 34卷 / 05期
关键词：
D O I：
10.1046/j.1472-765X.2002.01096.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains). Methods and Results: On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With a combination of these three enzymes, 22 types were found. In human strains, the most frequently occurring types were Cj.4 (28%), Cj.1 (19%), Cj. 13 (13%) and Cj. 2 (5%). In the case of poultry strains, the most frequent types were Cj. 1 (34%), Cj. 11 (22%), C.j. 21 (16%) and Cj. 15 (11%). Conclusions: The findings support the hypothesis that poultry is a significant source but not sole source of Campylobacter sp. in relation to humans. Significance and Impact of the Study: The typing of Campylobacter sp. forms the basis for an evaluation of the current state and risk assessment of various Campylobacter sp. sources in relation to humans.
引用
收藏
页码：354 / 358
页数：5
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