Differences in the Elemental Isotope Definition May Lead to Errors in Modern Mass-Spectrometry-Based Proteomics

被引：6
|
作者：
Claesen, Jurgen [1 ]
Lermyte, Frederik [2 ,3 ]
Sobott, Frank [2 ,3 ]
Burzykowski, Tomasz [1 ]
Valkenborg, Dirk [1 ,3 ,4 ]
机构：
[1] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, B-3590 Diepenbeek, Belgium
[2] Univ Antwerp, Biomol & Analyt Mass Spectrometry, B-2020 Antwerp, Belgium
[3] Univ Antwerp, Ctr Prote, B-2020 Antwerp, Belgium
[4] Vlaamse Instelling Technol Onderzoek, Appl Bio & Mol Syst, B-2400 Mol, Belgium
来源：
ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 21期
基金：
比利时弗兰德研究基金会;
关键词：
PEPTIDE; ACCURACY;
D O I：
10.1021/acs.analchem.5b01165
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The elemental isotope definition used to calculate the theoretical masses and isotope distribution of (bio)molecules is considered to be a fixed, universal standard in mass-spectrometry-based proteomics. However, this is an incorrect assumption. In view of the ongoing advances in mass spectrometry technology, and in particular the ever-increasing mass precision, the elemental isotope definition and its variations should be taken into account. We illustrate the effect of the elemental isotope uncertainty on the theoretical and experimental masses with theoretical calculations and examples.
引用
收藏
页码：10747 / 10754
页数：8
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