Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability

被引：9
|
作者：
Terracciano, Monica [1 ,2 ]
Rea, Ilaria [1 ]
Stefano, Luca De [1 ]
Rendina, Ivo [1 ]
Oliviero, Giorgia [2 ]
Nici, Fabrizia [2 ]
D'Errico, Stefano [2 ]
Piccialli, Gennaro [2 ]
Borbone, Nicola [2 ]
机构：
[1] Inst Microelectron & Microsystems, Natl Res Council, I-80131 Naples, Italy
[2] Univ Naples Federico II, Dept Pharm, I-80138 Naples, Italy
来源：
NANOSCALE RESEARCH LETTERS | 2014年 / 9卷
关键词：
Mesoporous silicon functionalization; DNA synthesis; Deprotection conditions; Surface stability; POROUS SILICON; BIOSENSORS; SURFACE;
D O I：
10.1186/1556-276X-9-317
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.
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页码：1 / 9
页数：9
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