Diversity analysis of pea genotypes using RAPD markers

被引：5
|
作者：
Thakur, Bharti [1 ]
Sharma, Susheel [1 ]
Sharma, Isha [1 ]
Sharma, Prachi [1 ]
Deepika [1 ]
Zargar, Sajad Majeed [1 ]
机构：
[1] Sher E Kashmir Univ Agr Sci & Technol, Div Plant Pathol, Jammu 180009, Jammu & Kashmir, India
来源：
LEGUME RESEARCH | 2018年 / 41卷 / 02期
关键词：
Genetic diversity; Pisum sativum; RAPD; GENETIC DIVERSITY; L; VARIETIES; CULTIVARS;
D O I：
10.18805/LR-3709
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Genetic diversity among 54 genotypes of field and garden pea of India were studied using 30 RAPD primers. A total of 168 amplicons were scored, of which 154 were polymorphic revealing 89.3% of polymophism. Out of 30 RAPD primers tested, 22 primers produced clear and reproducible bands. The number of products generated by these primers ranged from 04 to 14 with primer OPU-17 giving maximum (14) and primer OPC-08 giving minimum number of amplicons (04). Genetic similarity estimates based on the binomial data using Jaccard's coefficient ranged from 0.34 (Kaza-2/IC-218991) to 0.89 (IC-209118/IC-209123) with an average similarity index of 0.54 exhibiting considerable diversity among the pea genotypes studied. The dendrogram showed clear pattern of clustering according to the source of germplasm. This study indicated that Himalayan region germplasm exhibited higher diversity.
引用
收藏
页码：196 / 201
页数：6
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