Diversity analysis of pea genotypes using RAPD markers

被引：5
|
作者：
Thakur, Bharti [1 ]
Sharma, Susheel [1 ]
Sharma, Isha [1 ]
Sharma, Prachi [1 ]
Deepika [1 ]
Zargar, Sajad Majeed [1 ]
机构：
[1] Sher E Kashmir Univ Agr Sci & Technol, Div Plant Pathol, Jammu 180009, Jammu & Kashmir, India
来源：
LEGUME RESEARCH | 2018年 / 41卷 / 02期
关键词：
Genetic diversity; Pisum sativum; RAPD; GENETIC DIVERSITY; L; VARIETIES; CULTIVARS;
D O I：
10.18805/LR-3709
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Genetic diversity among 54 genotypes of field and garden pea of India were studied using 30 RAPD primers. A total of 168 amplicons were scored, of which 154 were polymorphic revealing 89.3% of polymophism. Out of 30 RAPD primers tested, 22 primers produced clear and reproducible bands. The number of products generated by these primers ranged from 04 to 14 with primer OPU-17 giving maximum (14) and primer OPC-08 giving minimum number of amplicons (04). Genetic similarity estimates based on the binomial data using Jaccard's coefficient ranged from 0.34 (Kaza-2/IC-218991) to 0.89 (IC-209118/IC-209123) with an average similarity index of 0.54 exhibiting considerable diversity among the pea genotypes studied. The dendrogram showed clear pattern of clustering according to the source of germplasm. This study indicated that Himalayan region germplasm exhibited higher diversity.
引用
收藏
页码：196 / 201
页数：6
相关论文
共 50 条
  • [1] Analysis of molecular diversity in okra (Abelmoschus esculentus) genotypes using RAPD markers
    Goswami, Aakansha
    Singh, B.
    Sharma, Anubhuti
    [J]. INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 2016, 86 (10): : 1310 - 1315
  • [2] A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers
    Souframanien, J
    Gopalakrishna, T
    [J]. THEORETICAL AND APPLIED GENETICS, 2004, 109 (08) : 1687 - 1693
  • [3] A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers
    J. Souframanien
    T. Gopalakrishna
    [J]. Theoretical and Applied Genetics, 2004, 109 : 1687 - 1693
  • [4] Genetic diversity analysis of marigold (Tagetes sp) genotypes using RAPD and ISSR markers
    Namita
    Panwar, Sapna
    Sonah, Humira
    Singh, Kanwar P.
    Sharma, T. R.
    [J]. INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 2013, 83 (05): : 8 - 14
  • [5] Fingerprint and genetic diversity analysis of Eucalyptus spp. genotypes using RAPD and SSR markers
    Rocha, Rodrigo Barros
    Muro Abad, Júpiter Israel
    Pires, Ismael Eleotério
    De Araújo, Elza Fernandes
    [J]. Scientia Forestalis/Forest Sciences, 2002, (62): : 24 - 31
  • [6] Genetic diversity of mulberry (Morus spp.) genotypes using RAPD markers
    Shabir, Wani A.
    Ashraf, Bhat M.
    Afifa, Kamili S.
    Nawsheeba, Wani
    Sabeena, Akhter
    Naina, Majid
    [J]. RESEARCH JOURNAL OF BIOTECHNOLOGY, 2014, 9 (06): : 41 - 47
  • [7] GENETIC DIVERSITY AMONG BREAD WHEAT GENOTYPES USING RAPD AND SSR MARKERS
    Al-Tamimi, Attyaf J. T.
    Al-Janabi, Ali S.
    [J]. SABRAO JOURNAL OF BREEDING AND GENETICS, 2019, 51 (03): : 325 - 339
  • [8] DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers
    Afridi, Sahib Gul
    Ahmad, Habib
    Alam, Mukhtar
    Khan, Imtiaz Ahmed
    Hassan, Mukhtiar
    [J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (69): : 15477 - 15482
  • [9] GENETIC DIVERSITY STUDIES AMONG COLOURED COTTON GENOTYPES BY USING RAPD MARKERS
    Khan, Azeem Iqbal
    Awan, Faisal Saeed
    Sadia, Bushra
    Rana, Rashid Mehmood
    Khan, Iftikhar Ahmad
    [J]. PAKISTAN JOURNAL OF BOTANY, 2010, 42 (01) : 71 - 77
  • [10] Analysis of molecular diversity in guava using RAPD markers
    Prakash, DP
    Narayanaswamy, P
    Sondur, SN
    [J]. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2002, 77 (03): : 287 - 293
12345