Modification of the dynamic nature of the chimeric fumarate two-component system in Escherichia coli via positive feedback loop

被引：1

作者：

Ganesh, Irisappan ^{[1
]}

Maruthamuthu, Murali Kannan ^{[1
]}

Yoo, Ik-keun ^{[1
]}

Hong, Soon Ho ^{[1
]}

机构：

[1] Univ Ulsan, Dept Chem Engn, Ulsan 680749, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2015年 / 20卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

fumarate; OmpR; positive feedback loop; two-component system; Escherichia coli;

D O I：

10.1007/s12257-015-0255-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A positive feedback loop was introduced to modify the dynamic behavior of fumarate sensing DcuSZ chimera TCS. To construct the positive feedback loop, the ompR gene was cloned downstream of the ompC promoter. The ompC promoter induced the expression of OmpR, which in turn induced the expression of the ompC promoter. Through the introduction of this positive feedback loop, the transcriptional expression levels of ompC increased 2.6-fold. When GFP was used as a reporter protein, a 64% increase in fluorescence level was observed. These results suggest that sensitivity of the TCS based fumarate sensing system can be engineered through the introduction of a positive feedback loop.

引用

页码：844 / 848

页数：5

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