Species differences in bacterial NhaA Na+/H+ exchangers

被引：15

作者：

Calinescu, Octavian ^{[1
]}

Danner, Eva ^{[1
]}

Boehm, Marc ^{[2
]}

Hunte, Carola ^{[3
]}

Fendler, Klaus ^{[1
]}

机构：

[1] Max Planck Inst Biophys, Dept Biophys Chem, D-60438 Frankfurt, Germany

[2] Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60438 Frankfurt, Germany

[3] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, ZBMZ, Inst Biochem & Mol Biol, D-79106 Freiburg, Germany

来源：

FEBS LETTERS | 2014年 / 588卷 / 17期

关键词：

NhaA; Cation/proton antiport; Solid supported membrane; Helicobacter pylori; Escherichia coli; Salmonella typhimurium; ANTIPORTER NHAA; CHARGE TRANSLOCATION; TRANSPORT MECHANISM; HELICOBACTER-PYLORI; PH REGULATION; EXPRESSION; PROTEIN; CLONING; COLI;

D O I：

10.1016/j.febslet.2014.05.066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteria have adapted their NhaA Na+/H+ exchangers responsible for salt homeostasis to their different habitats. We present an electrophysiological and kinetic analysis of NhaA from Helicobacter pylori and compare it to the previously investigated exchangers from Escherichia coli and Salmonella typhimurium. Properties of all three transporters are described by a simple model using a single binding site for H+ and Na+. We show that H. pylori NhaA only has a small acidic shift of its pH-dependent activity profile compared to the other transporters and discuss why a more drastic change in its pH activity profile is not physiologically required. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

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页码：3111 / 3116

页数：6

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