Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina

被引：40
|
作者：
Belli, Sabina I. [1 ]
Ferguson, David J. P. [2 ]
Katrib, Marilyn [1 ]
Slapetova, Iveta [1 ]
Mai, Kelly [1 ]
Slapeta, Jan [1 ]
Flowers, Sarah A. [1 ]
Miska, Kate B. [3 ]
Tomley, Fiona M. [4 ]
Shirley, Martin W. [4 ]
Wallach, Michael G. [1 ]
Smith, Nicholas C. [1 ]
机构：
[1] Univ Technol Sydney, Inst Biotechnol Infect Dis, Sydney, NSW 2007, Australia
[2] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Pathol, Oxford OX3 9DU, England
[3] Agr Res Serv, USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA
[4] Inst Anim Hlth, Newbury RG20 7NN, Berks, England
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2009年 / 39卷 / 10期
基金：
英国惠康基金; 澳大利亚研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词：
Eimeria; Apicomplexa; Coccidiosis; Macrogametocyte; Oocyst wall; Immunolocalisation; GAMETOCYTE ANTIGENS; COCCIDIAN PARASITE; CHICKENS; INFECTION; VACCINE; GAM56;
D O I：
10.1016/j.ijpara.2009.05.004
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Vaccination with proteins from gametocytes of Eimeria maxima protects chickens, via transfer of maternal antibodies, against infection with several species of Eimeria. Antibodies to E. maxima gametocyte proteins recognise proteins in the wall forming bodies of macrogametocytes and oocyst walls of E. maxima, Eimeria tenella and Eimeria acervulina. Homologous genes for two major gametocyte proteins - GAM56 and GAM82 - were found in E. maxima, E. tenella and E. acervulina. Alignment of the predicted protein sequences of these genes reveals that, as well as sharing regions of tyrosine richness, strong homology exists in their ammo-terminal regions, where protective antibodies bind. This Study confirms the conservation of the roles of GAM56 and GAM82 in oocyst wall formation and shows that antibodies to gametocyte antigens of E. maxima cross-react with homologous proteins in other species, helping to explain cross-species maternal immunity. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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页码：1063 / 1070
页数：8
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