Electron microscopic cytochemistry of adenylyl cyclase activity in mouse spermatozoa

被引:8
|
作者
Liguori, L
Rambotti, MG
Bellezza, L
Minelli, A
机构
[1] Univ Perugia, Sez Anat Umana, Sez Biochim Cellulare, Dipartimento Sci Biochim Biotecnol Mol, I-06123 Perugia, Italy
[2] Univ Perugia, Sez Anat Umana, Dipartimento Med Sperimentale, I-06123 Perugia, Italy
关键词
adenylyl cyclase; mouse spermatozoa localization; electron microscopy;
D O I
10.1369/jhc.3B6141.2004
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We investigated adenylyl cyclase activity of mouse spermatozoa by electron microscopic cytochemistry. Subcellular localization of enzyme activity was determined in the presence and absence of bicarbonate ions. Results confirm the existence in sperm of a bicarbonate-regulated adenylyl cyclase, which suggests microdomain signaling.
引用
收藏
页码:833 / 836
页数:4
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