A soluble adenylyl cyclase from sea urchin spermatozoa

被引:37
|
作者
Nomura, M [1 ]
Beltrán, C
Darszon, A
Vacquier, VD
机构
[1] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA
[2] Univ Nacl Autonoma Mexico, Dept Genet & Fisiol Mol, Cuernavaca 62250, Morelos, Mexico
关键词
sperm motility; invertebrate fertilization; cyclic nucleotides; flagella; cAMP; metabolism in sperm;
D O I
10.1016/j.gene.2005.04.034
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A previously identified, calmodulin-binding, sea urchin sperm flagellar adenylyl cyclase (AC) was cloned and sequenced and found to be a homologue of mammalian sperm soluble adenylyl cyclase (sAC). Compared to the mammalian sAC, the sea urchin sAC (susAC) has several long amino acid insertions, some of which contain protein kinase A phosphorylation sites. The enzymatic activity of susAC shows a steep pH dependency curve, the specific activity doubling when the pH is increased from 7.0 to 7.5. This suggests that like sperm dynein ATPase, the susAC is probably activated by increases in intracellular pH occurring upon spawning into seawater and also when sperm respond to contact with the egg jelly layer. The susAC is strongly activated by manganese, but has low activity in magnesium. Gene database searches identified sAC homologues in species known to have cyclic AMP-dependent sperm motility. This implies (as shown in mouse) that susAC has a role in sperm motility, most probably through axonemal protein phosphorylation or ion channel regulation. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:231 / 238
页数:8
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