A colorimetric assay for screening transketolase activity

被引：45
|
作者：
Smith, Mark E. B.
Kaulmann, Ursula
Ward, John M.
Hailes, Helen C.
机构：
[1] UCL, Dept Chem, London WC1H 0AJ, England
[2] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2006年 / 14卷 / 20期
基金：
英国工程与自然科学研究理事会;
关键词：
colorimetric assay; transketolase; directed evolution; tetrazolium red;
D O I：
10.1016/j.bmc.2006.06.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A tetrazolium red-based colorimetric assay has been devised to screen for transketolase activity with a range of aldehyde acceptors. The colorimetric TK assay is able to detect > 8% bioconversion using non-alpha-hydroxylated aldehydes as acceptor substrates and is significantly faster and more convenient to use than chromatographic procedures. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：7062 / 7065
页数：4
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