Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization

被引：11
|
作者：
Kakita, Hirotaka
Kamishima, Hiroshi
Inouye, Kuniyo
机构：
[1] Natl Inst Adv Ind Sci & Technol, AIST Shikoku, Takamatsu, Kagawa 7610395, Japan
[2] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Sakyo Ku, Kyoto 6068502, Japan
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1129卷 / 02期
关键词：
uronic acid; fluorimetric determination; microanalysis; postcolumn derivatization; normal phase partition chromatography; HPLC;
D O I：
10.1016/j.chroma.2006.08.057
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
To develop a fluorimetric high performance liquid chromatography (HPLC) technique for uronic acid microanalysis, a saline mobile phase and the postcolumn fluorimetric determination were combined. The detection limits Of D-glucuronic, D-galacturonic and D-mannuronic acids were 7.19, 23.88 and 7.08 pmol, respectively. The proposed method was successfully applied to uronic acid n-ticroanalysis in a polysaccharide hydrolysate and a drink. (c) 2006 Elsevier B.V. All rights reserved.
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页码：296 / 299
页数：4
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