DETERMINATION OF PSEUDOURIDINE IN HUMAN URINE AND SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN FLUORESCENCE DERIVATIZATION

被引：6
|
作者：
UMEGAE, Y [1 ]
NOHTA, H [1 ]
OHKURA, Y [1 ]
机构：
[1] KYUSHU UNIV 62,FAC PHARMACEUT SCI,HIGASHI KU,FUKUOKA 812,JAPAN
来源：
JOURNAL OF CHROMATOGRAPHY | 1990年 / 515卷
关键词：
D O I：
10.1016/S0021-9673(01)89345-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective and sensitive method for the determination of psuedouridine in human urine and serum is described. The method is based on high-performance liquid chromatography with post-column fluorescence derivatization. Pseudouridine and 5-fluorouridine (internal standard) in a 10-fold diluted urine sample or a deproteinized serum sample are separated on a reversed-phase column (TSK gel ODS-80TM) with isocratic elution and successively subjected to derivatization involving periodate oxidation followed by fluorescence reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine. The detection limit for pseudouridine is 4 pmol in a 100-μl injection volume. © 1990.
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页码：495 / 501
页数：7
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