Application of MALDI-TOF MS Systems in the Rapid Identification of Campylobacter spp. of Public Health Importance

被引:14
|
作者
Hsieh, Ying-Hsin [1 ]
Wang, Yun F. [2 ,3 ]
Moura, Hercules [4 ]
Miranda, Nancy [1 ]
Simpson, Steven [1 ]
Gowrishankar, Ramnath [4 ]
Barr, John [4 ]
Kerdahi, Khalil [1 ]
Sulaiman, Irshad M. [1 ]
机构
[1] US FDA, Microbiol Sci Branch, Southeast Food & Feed Lab, 60 8th St NE, Atlanta, GA 30309 USA
[2] Emory Univ, Sch Med, 1364 Clifton Rd NE, Atlanta, GA 30322 USA
[3] Grady Mem Hosp, Clin Pathol Lab, 80 Jesse Hill Dr, Atlanta, GA 30303 USA
[4] US Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, Div Lab Sci, Clin Chem Branch, 4770 Buford Highway NE, Atlanta, GA 30341 USA
关键词
DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; GENETIC-CHARACTERIZATION; FOODBORNE PATHOGENS; BRUKER BIOTYPER; BACTERIA; JEJUNI; FOOD;
D O I
10.5740/jaoacint.17-0266
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Campylobacteriosis is an infectious gastrointestinal disease caused by Campylobacter spp. In most cases, it is either underdiagnosed or underreported due to poor diagnostics and limited databases. Several DNA-based molecular diagnostic techniques, including 16S ribosomal RNA (rRNA) sequence typing, have been widely used in the species identification of Campylobacter. Nevertheless, these assays are time-consuming and require a high quality of bacterial DNA. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS is an emerging diagnostic technology that can provide the rapid identification of microorganisms by using their intact cells without extraction or purification. In this study, we analyzed 24 American Type Culture Collection reference isolates of 16 Campylobacter spp. and five unknown clinical bacterial isolates for rapid identification utilizing two commercially available MADI-TOF MS platforms, namely the bioMerieux VITEK (R) MS and Bruker Biotyper systems. In addition, 16S rRNA sequencing was performed to confirm the species-level identification of the unknown clinical isolates. Both MALDI-TOF MS systems identified the isolates of C. jejuni, C. coli, C. lad, and C. fetus. The results of this study suggest that the MALDI-TOF MS technique can be used in the identification of Campylobacter spp. of public health importance.
引用
收藏
页码:761 / 768
页数:8
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