Purification and Characterization of Camel (Camelus dromedarius) Milk Amylase

Skimmed camel milk contains 59,900U/L amylase, which is 39,363 times less than serum and plasma amylase. Camel milk -amylase was purified as a 61KDa band using DEAE-Sepharose and Sephadex G-100 and yielded 561U/mg. The optimum working pH, Km and temperature were 7.0, 13.6mg/Lstarch, 30-40C, respectively. The enzyme has been shown higher affinity toward amylose and soluble starch than glycogen, amylopectin, dextrin, or pullulan. Magnesium chloride, CaCl2 and NaCl activated the amylase, while EDTA and EGTA decreased its activity. While its activity was increased in the presence of Triton X-100 and Triton X-114. Phenylmethanesulfonyl fluoride did not show any effect on enzyme activity. However, the enzyme activity was inhibited by urea, SDS, DTNB, iodoacetamide, N-ethylmalimide, aprotinin, and trypsin inhibitor. It worked on starch to yield a maltose. Scanning electron microscope images demonstrated a nano-degrading ability on starch granules from various sources (potato, corn, cassava, and rice).