Refinement and Extension of AOAC Method 2005.06 to Include Additional Toxins in Mussels: Single-Laboratory Validation

被引：1
|
作者：
Turner, Andrew D. [1 ]
Norton, Deirdre M. [1 ]
Hatfield, Robert G. [1 ]
Morris, Steven [1 ]
Reese, Allan R. [1 ]
Algoet, Myriam [1 ]
Lees, David N. [1 ]
机构：
[1] Ctr Environm Fisheries & Aquaculture Sci, Weymouth DT4 8UB, Dorset, England
来源：
JOURNAL OF AOAC INTERNATIONAL | 2009年 / 92卷 / 01期
关键词：
SHELLFISH POISONING TOXINS; LIQUID-CHROMATOGRAPHIC DETERMINATION; PARALYTIC SHELLFISH; PRECHROMATOGRAPHIC OXIDATION; QUANTITATIVE-DETERMINATION; FLUORESCENCE DETECTION;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A single-laboratory validation study was undertaken for the analysis of paralytic shellfish poisoning (PSP) toxins in common mussels, extending AOAC Official Method 2005.06 to include the additional toxins dcNEO and dcGTX2,3. The method was refined to improve toxin oxidation product stability; analytical sensitivity of N-hydroxylated toxins, and throughput. Validation was performed to characterize the method for selectivity, sensitivity, linearity, precision, repeatability, recovery, ruggedness, and uncertainty. Parallel testing of naturally contaminated mussels enabled comparison of sample toxicities obtained using mouse bioassay (MBA) and high-performance liquid chromatographic (HPLC) methodologies. Performance characteristics of the method are reported for all commercially available certified reference toxins. Results from the MBA and HPLC methods were well correlated, and the analytical method has been instigated as the sole monitoring tool for UK official control surveillance of PSP toxins in common mussels.
引用
收藏
页码：190 / 207
页数：18
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