Visual evidence of nitrate reductase exudation from plant roots

To prove the nitrate reductase exudation, roots of 22 plant species were kept in contact with filter sheet impregnated with N-(1-naphthyl)ethyl-diamine dihydrochloride, sulphanilamide and potassium nitrate, Nitrate reductase exudation was indicated by the appearance of root impressions in red colour. Nitrate reductase exudation was observed from the roots of 13 plant species even though roots of all species showed its activity.