Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis

被引：19
|
作者：
Kefala, Georgia [1 ]
Weiss, Manfred S. [1 ]
机构：
[1] DESY, EMBL Hamburg Outstn, D-22603 Hamburg, Germany
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷
关键词：
D O I：
10.1107/S1744309106039844
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dihydrodipicolinate synthase from Mycobacterium tuberculosis (DHDPS, DapA, Rv2753c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in a monoclinic crystal form. Preliminary diffraction data analysis suggests the presence of two independent tetramers in the asymmetric unit in almost the same relative orientation.
引用
收藏
页码：1116 / 1119
页数：4
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