Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis

被引：9
|
作者：
Kefala, G [1 ]
Janowski, R [1 ]
Panjikar, S [1 ]
Mueller-Dieckmann, C [1 ]
Weiss, MS [1 ]
机构：
[1] DESY, EMBL Hamburg Outstn, D-22603 Hamburg, Germany
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S174430910501938X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.
引用
收藏
页码：718 / 721
页数：4
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