Impact of Lifelong Sedentary Behavior on Mitochondrial Function of Mice Skeletal Muscle

This study investigated the impact of lifelong sedentariness oil skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y: old sedentary-M old activc-OA). Young animals were sacrificed after I week of quarantine. and OS and OA groups were individually placed into Standard calves and in cages with running wheels. respectively, until sacrifice (25 months). Body weights and hind-limb skeletal Muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e. state 3 and 4 respiration, respiratory control ratio. and ratio of nanomoles of ADP phosphorylated by nanomoles of O-2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity. protein carbonyl derivatives. sulfhydryl groups) were Measured in isolated mitochondrial Suspensions. Our results reveal that lifelong, sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and oil the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.