Gateway vectors for plant transformation

被引：75
|
作者：
Nakagawa, Tsuyoshi [1 ]
Ishiguro, Sumie [2 ]
Kimura, Tetsuya [3 ]
机构：
[1] Shimane Univ, Dept Mol & Funct Genom, Ctr Integrated Res Sci, Matsue, Shimane 6908504, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Dept Biol Mech & Funct, Nagoya, Aichi 4648601, Japan
[3] Mie Univ, Grad Sch Bioresources, Dept Sustainable Resource Sci, Tsu, Mie 5148507, Japan
来源：
PLANT BIOTECHNOLOGY | 2009年 / 26卷 / 03期
关键词：
Binary vector; epitope tag; Gateway cloning; reporter; BINARY VECTORS; RECOMBINATIONAL CLONING; FUNCTIONAL GENOMICS; DNA CLONING; GENE; VERSATILE; CONSTRUCTS;
D O I：
10.5511/plantbiotechnology.26.275
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The availability of complete plant genome sequences has opened a new era of plant molecular research using approaches such as comprehensive expression analysis, ectopic expression and gene knockdown. For Such purposes, new tools realizing easy and rapid construction of recombinant plasmids are required. Here, we describe Gateway cloning compatible binary vectors for post-genomic research in plant genetic engineering. The vectors comprise a variety of reporters, epitope tags and selective markers that should make them useful for construction of plasmids for Agrobacterium-mediated transformation of plants. We also describe vectors for promoter swapping using Gateway cloning technology.
引用
收藏
页码：275 / 284
页数：10
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