Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation

被引：148

作者：

Nakamura, Shinya ^{[2
]}

Mano, Shoji ^{[3
,4
]}

Tanaka, Yuji ^{[2
]}

Ohnishi, Masato ^{[2
]}

Nakamori, Chihiro ^{[3
]}

Araki, Masami ^{[3
]}

Niwa, Tomoko ^{[1
]}

Nishimura, Mikio ^{[3
,4
]}

Kaminaka, Hironori ^{[5
]}

Nakagawa, Tsuyoshi ^{[2
]}

Sato, Yutaka ^{[1
,6
]}

Ishiguro, Sumie ^{[1
]}

机构：

[1] Nagoya Univ, Dept Biol Mech & Funct, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

[2] Shimane Univ, Dept Mol & Funct Genom, Ctr Integrated Res Sci, Matsue, Shimane 6908504, Japan

[3] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan

[4] Grad Univ Adv Studies, Dept Basic Biol, Sch Life Sci, Okazaki, Aichi 4448585, Japan

[5] Tottori Univ, Dept Bioresource Sci, Fac Agr, Tottori 6808553, Japan

[6] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2010年 / 74卷 / 06期

基金：

日本学术振兴会;

关键词：

bialaphos resistance gene (bar); binary vector; Gateway cloning; glufosinate; plant transformation; PROTEIN; ARABIDOPSIS; AGROBACTERIUM; PURIFICATION; EFFICIENT; MUTANTS; FUSIONS; COMPLEX; CLONING; GFP;

D O I：

10.1271/bbb.100184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA (R) was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA (R) or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

引用

页码：1315 / 1319

页数：5

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