Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting

被引：3
|
作者：
Araya, Carolina M. [1 ,2 ]
Cazorla, Alberto [3 ,4 ]
Reche, Isabel [1 ,2 ,5 ]
机构：
[1] Univ Granada, Dept Ecol, Fac Ciencias, E-18071 Granada, Spain
[2] Univ Granada, Inst Agua, Fac Ciencias, E-18071 Granada, Spain
[3] Univ Granada, Dept Appl Phys, E-18071 Granada, Spain
[4] IISTA CEAMA, Andalusian Inst Earth Syst Res, Granada, Spain
[5] Univ Granada, Res Unit Modeling Nat MNat, Granada, Spain
来源：
BIO-PROTOCOL | 2019年 / 9卷 / 12期
关键词：
Airborne bacteria; Bioaerosol; Microbial dispersal; Cloud condensation nuclei; Ice condensation nuclei; CELLS;
D O I：
10.21769/BioProtoc.3273
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment procedure consists of a chemical treatment with sodium pyrophosphate and Tween 20 and a physical treatment with agitation and ultrasonic bath to disperse the bacteria in the liquid sample. We recover the total (free and attached) bacteria by generating a density gradient with Nycodenz by centrifugation. The techniques prior to this procedure do not include the microorganisms that are attached to the aerosol particles and, therefore, considerably underestimate the total load and deposition of airborne microorganisms.
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页数：9
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