Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

被引：443
|
作者：
Gota, Chie [1 ]
Okabe, Kohki [1 ,2 ]
Funatsu, Takashi [1 ,3 ]
Harada, Yoshie [2 ]
Uchiyama, Seiichi [1 ]
机构：
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1138613, Japan
[3] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 08期
关键词：
SINGLE LIVING CELLS; TEMPERATURE-MEASUREMENT; PHASE-TRANSITIONS; THERMOGENESIS; MICROGELS; POLYMERS; POLARITY; PROBES; VOLUME; HEAT;
D O I：
10.1021/ja807714j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.
引用
收藏
页码：2766 / +
页数：3
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