A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

被引：69

作者：

Uchiyama, Seiichi ^{[1
]}

Tsuji, Toshikazu ^{[1
,2
]}

Ikado, Kumiko ^{[2
]}

Yoshida, Aruto ^{[2
]}

Kawamoto, Kyoko ^{[1
]}

Hayashi, Teruyuki ^{[3
]}

Inada, Noriko ^{[3
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] KIRIN Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[3] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300192, Japan

来源：

ANALYST | 2015年 / 140卷 / 13期

关键词：

SINGLE LIVING CELLS; IMAGING MICROSCOPY; DNA; NANOTHERMOMETERS; SPECTROSCOPY; LIFETIME; MOLT-4; PROBES; CYCLE;

D O I：

10.1039/c5an00420a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01-1.0 degrees C) using the new cationic fluorescent polymeric thermometer.

引用

页码：4498 / 4506

页数：9

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