Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis

被引：602

作者：

Funamoto, S ^{[1
]}

Meili, R ^{[1
]}

Lee, S ^{[1
]}

Parry, L ^{[1
]}

Firtel, RA ^{[1
]}

机构：

[1] Univ Calif San Diego, Ctr Mol Genet, Div Biol, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

来源：

CELL | 2002年 / 109卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(02)00755-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the mechanisms of leading edge formation in chemotaxing Dictyostelium cells. We demonstrate that while phosphatidylinositol 3-kinase (PI3K) transiently translocates to the plasma membrane in response to chemoattractant stimulation and to the leading edge in chemotaxing cells, PTEN, a negative regulator of PI3K pathways, exhibits a reciprocal pattern of localization. By uniformly localizing PI3K along the plasma membrane, we show that chemotaxis pathways are activated along the lateral sides of cells and PI3K can initiate pseudopod formation, providing evidence for a direct instructional role of PI3K in leading edge formation. These findings provide evidence that differential subcellular localization and activation of PI3K and PTEN is required for proper chemotaxis.

引用

页码：611 / 623

页数：13

共 50 条

[31] A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy
Jao, Christine C.
Ragusa, Michael J.
Stanley, Robin E.
Hurley, James H.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (14) : 5486 - 5491
[32] Regulation of breast cancer cell chemotaxis by the phosphoinositide 3-kinase p110δ
Sawyer, C
Sturge, J
Bennett, DC
O'Hare, MJ
Allen, WE
Bain, J
Jones, GE
Vanhaesebroeck, B
CANCER RESEARCH, 2003, 63 (07) : 1667 - 1675
[33] PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus
Ahn, JY
Rong, R
Liu, XS
Ye, KQ
EMBO JOURNAL, 2004, 23 (20): : 3995 - 4006
[34] Direct positive regulation of PTEN by the p85 subunit of phosphatidylinositol 3-kinase
Chagpar, Ryaz B.
Links, Philip H.
Pastor, M. Chris
Furber, Levi A.
Hawrysh, Andrea D.
Chamberlain, M. Dean
Anderson, Deborah H.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (12) : 5471 - 5476
[35] DISTINCT PHOSPHATIDYLINOSITOL 3-KINASE (PI 3-KINASE) ACTIVITIES FROM HUMAN-LYMPHOCYTES
VARTICOVSKI, L
KEELER, ML
WU, SS
SPRING, ME
FASEB JOURNAL, 1992, 6 (05): : A1923 - A1923
[36] The role of PI 3-kinase in insulin action
Alessi, DR
Downes, CP
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1998, 1436 (1-2): : 151 - 164
[37] PI 3-kinase, Akt and cell survival
Downward, J
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2004, 15 (02) : 177 - 182
[38] Role of PI 3-kinase in signal transduction
Cantley, L
Rameh, L
Toker, A
Franke, T
Kaplan, D
FASEB JOURNAL, 1997, 11 (09): : A1010 - A1010
[39] Biological roles of PI 3-kinase isoforms
Vanhaesebroeck, B.
EJC SUPPLEMENTS, 2008, 6 (12): : 4 - 4
[40] Flavonoids and the inhibition of PKC and PI 3-kinase
Gamet-Payrastre, L
Manenti, S
Gratacap, MP
Tulliez, J
Chap, H
Payrastre, B
GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM, 1999, 32 (03): : 279 - 286

← 1 2 3 4 5 →