The plant epitranscriptome: revisiting pseudouridine and 2′-O-methyl RNA modifications

被引：12

作者：

Ramakrishnan, Muthusamy ^{[1
,2
]}

Rajan, K. Shanmugha ^{[3
,4
,5
]}

Mullasseri, Sileesh ^{[6
]}

Palakkal, Sarin ^{[7
]}

Kalpana, Krishnan ^{[8
]}

Sharma, Anket ^{[9
]}

Zhou, Mingbing ^{[9
,10
]}

Vinod, Kunnummal Kurungara ^{[11
]}

Ramasamy, Subbiah ^{[12
]}

Wei, Qiang ^{[1
,2
]}

机构：

[1] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China

[2] Nanjing Forestry Univ, Bamboo Res Inst, Nanjing, Jiangsu, Peoples R China

[3] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel

[4] Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, Israel

[5] Weizmann Inst Sci, Dept Chem & Struct Biol, IL-7610001 Rehovot, Israel

[6] Kerala Univ Fisheries & Ocean Studies, Sch Ocean Sci & Technol, Cochin, Kerala, India

[7] Hebrew Univ Jerusalem, Sch Pharm, Inst Drug Res, Jerusalem, Israel

[8] Tamilnadu Agr Univ, Agr Coll & Res Inst, Dept Plant Pathol, Madurai 625104, Tamil Nadu, India

[9] Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China

[10] Zhejiang A&F Univ, Zhejiang Prov Collaborat Innovat Ctr Bamboo Resou, Hangzhou, Zhejiang, Peoples R China

[11] Indian Agr Res Inst, ICAR, Div Genet, New Delhi, India

[12] Madurai Kamaraj Univ, Sch Biol Sci, Dept Biochem, Cardiac Metab Dis Lab, Madurai, Tamil Nadu, India

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2022年 / 20卷 / 07期

关键词：

epitranscriptomics; pseudouridine; 2 '-O-methylation; RNA modifications; next-generation sequencing; ARABIDOPSIS MESSENGER-RNA; SITE RECOGNITION REGION; RIBOSOMAL-RNA; U2; SNRNA; FORM PSEUDOURIDINES; M(6)A DEMETHYLASE; MASS-SPECTROMETRY; RIBONUCLEIC-ACIDS; HIGH-THROUGHPUT; METHYLATION;

D O I：

10.1111/pbi.13829

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

There is growing evidence that post-transcriptional RNA modifications are highly dynamic and can be used to improve crop production. Although more than 172 unique types of RNA modifications have been identified throughout the kingdom of life, we are yet to leverage upon the understanding to optimize RNA modifications in crops to improve productivity. The contributions of internal mRNA modifications such as N6-methyladenosine (m(6)A) and 5-methylcytosine (m(5)C) methylations to embryonic development, root development, leaf morphogenesis, flowering, fruit ripening and stress response are sufficiently known, but the roles of the two most abundant RNA modifications, pseudouridine (Psi) and 2'-O-methylation (Nm), in the cell remain unclear due to insufficient advances in high-throughput technologies in plant development. Therefore, in this review, we discuss the latest methods and insights gained in mapping internal Psi and Nm and their unique properties in plants and other organisms. In addition, we discuss the limitations that remain in high-throughput technologies for qualitative and quantitative mapping of these RNA modifications and highlight future challenges in regulating the plant epitranscriptome.

引用

页码：1241 / 1256

页数：16

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