cypris major protein;
4-n-nonylphenol;
17;
beta-oestradiol;
barnacle;
endocrine disruption;
D O I:
10.1016/S0166-445X(99)00018-1
中图分类号:
Q17 [水生生物学];
学科分类号:
071004 ;
摘要:
Intense efforts are currently being made to develop procedures to assess the potential for endocrine disruption in aquatic environments. Most attention has focused on fresh water systems, amphibians and fish and morphological and molecular measures for endocrine disruption. A biomarker approach involving the induction of a female-specific protein, vitellogenin, in males by exposure to xeno-oestrogens has proved particularly useful. By contrast marine environments and invertebrates have received comparatively little attention. The present study aimed to examine the effects of exposure to a xeno-oetrogen, 4-n-nonylphenol (4-NP) and a natural oestrogen, 17 beta-oestradiol (E-2) on a marine crustacean, the barnacle Balanus amphitrite. In particular, the effect of such exposure on levels of a larval storage protein, cypris major protein (CMP), which is related to barnacle vitellin, has been examined. Accordingly, nauplius stage larvae of B. amphitrite were exposed to low concentrations (0.01-1.0 mu g l(-1)) of 4-NP and E-2 (1.0 mu g l(-1)) from egg hatching until the cypris stage. SDS-PAGE and immunoblotting were used to monitor CMP levels in exposed larvae relative to 'zero' concentration controls. Elevated (100% increase) CMP levels were measured in larvae exposed to both 4-NP and E-2 at a concentration of 1.0 mu g l(-1), and notable increases occurred at lower concentrations of 4-NP. It is concluded that CMP and perhaps other vitellin-like proteins are potential biomarkers of low level oestrogen exposure in crustaceans. (C) 2000 Elsevier Science B.V. All rights reserved.
机构:
South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Ding, Na
Wang, Lu
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South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Wang, Lu
Kang, Yuan
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机构:
South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China
South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Kang, Yuan
Luo, Kesong
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South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Luo, Kesong
Zeng, Diya
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South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Zeng, Diya
Man, Yu Bon
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机构:
Educ Univ Hong Kong, CHEER, Hong Kong, Peoples R China
Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Man, Yu Bon
Zhang, Qiuyun
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South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China
South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Zhang, Qiuyun
Zeng, Lixuan
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机构:
South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China
South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Zeng, Lixuan
Luo, Jiwen
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South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China
South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
Luo, Jiwen
Jiang, Feng
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机构:
South China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China
South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R ChinaSouth China Normal Univ, Higher Educ Mega Ctr, Sch Environm, Guangzhou 510006, Peoples R China
机构:
Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
Andrew, M. N.
Dunstan, R. H.
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Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
Dunstan, R. H.
O'Connor, W. A.
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机构:
Port Stephens Res Ctr, New S Wales Dept Primary Ind, Taylors Beach, NSW 2316, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
O'Connor, W. A.
Van Zwieten, L.
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Environm Ctr Excellence, New S Wales Dept Primary Ind, Wollongbar, NSW 2477, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
Van Zwieten, L.
Nixon, B.
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Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
Nixon, B.
MacFarlane, G. R.
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Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, AustraliaUniv Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia