Isolation and characterization of beta-hydroxybutyrate dehydrogenase-deficient mutant of Rhodobacter sphaeroides 2.4.1

被引：0
|
作者：
Kho, DH [1 ]
Lee, JK [1 ]
机构：
[1] SOGANG UNIV,DEPT LIFE SCI,MAPO GU,SEOUL 121742,SOUTH KOREA
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 1997年 / 7卷 / 05期
关键词：
Rhodobacter spaeroides; beta-hydroxybutyrate dehydrogenase; leaky mutant; Tn5;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A transposon Tn5 mutant of Rhodobacter sphaeroides 2.4.1 was isolated for its impaired ability of growth on minimal medium containing P-hydroxybutyric acid as a sole carbon source. The mutant, R. sphaeroides S7 showed approximately 6-fold decrease in P-hydroxybutyrate dehydrogenase activity compared with that of wild type. in R. sphaeroides S7 the Tn5 was located in DNA region corresponding to a 4.2-kb EcoRI DNA fragment of R. sphaeroides 2.4.1 chromosome.
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页码：360 / 362
页数：3
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