Isolation, Identification and Molecular Characterization of Cellulolytic Bacteria from Rumen Samples collected from Erzurum Slaughter House, Turkey

In this study, the isolation, identification and molecular characterization of 36 bacteria samples of Achromobacter, Bacillus, Brevibacillus, Citrobacter, Clostridium, Enterococcus, Escherichia, Exiguobacterium, Lactobacillus, Lactococcus, Macrococcus, Neisseria, Ochrobactrum, Paenibacillus, Shigella,, Staphylococcus, Streptococcus and Streptomyces genera, which show cellulolytic properties, were performed from the rumen samples taken from the slaughterhouses in Erzurum. For this reason, the salt, pH and temperature demands of these isolates, which were considered to be different from each other and which showed cellulolytic properties were determined. Upon the analyses it was determined that the salt ratio in which the test strains could develop best was 2-4% and the pH and temperature range was between 5-9 and 15-37 degrees C respectively. It was also observed that only the strain with the code OZK24 from the cellulolytic bacteria could develop at 13% salt and pH 11 values. In addition, the 16S rRNA sequenceanalyses of the test bacteria were performed and it was observed that the OZK21 strain showed similarity to Macrococus caseolyticus at a rate of 98% and the OZK30 strain showed 97% similarity to Clostridium magnum. According to these data at hand, it was concluded that the potential of the two isolates being new species was high.