Isolation and Identification of Cellulolytic and Ethanologenic Bacteria from Soil

被引:6
|
作者
Chaudhary, Naureen [1 ]
Qazi, Javed Iqbal [1 ]
Irfan, Muhammad [2 ]
机构
[1] Univ Punjab, Dept Zool, Microbial Biotechnol Lab, Lahore, Pakistan
[2] Univ Sargodha, Dept Biotechnol, Sargodha, Pakistan
关键词
Isolation; Identification; 16S rRNA; Cellulase; Ethanol; Bacteria;
D O I
10.1007/s40995-017-0282-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, five different strains of bacteria were isolated and identified from soil samples cultivated on sugarcane bagasse as substrate in submerged fermentation. These bacterial isolates were identified by 16S rRNA gene sequencing which revealed that they were Bacillus pumilis, Bacillus licheniformis, Paenibacillus dendritiformis, Bacillus cereus and Pseudomonas aeruginosa. These bacteria were screened for cellulolytic and ethanologenic potentials using sugarcane bagasse as substrate. All the bacteria were found cellulolytic and ethanologenic. Bacillus pumilis strain produced maximum cellulase while B. cereus yielded maximum ethanol. The results indicated that these strains could be used for second generation biofuel production.
引用
收藏
页码:551 / 555
页数:5
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