ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL AND EXTRACTS OF FERULA COMMUNIS AND DETERMINATION OF CHEMICAL COMPOSITION OF ITS ESSENTIAL OIL

In this study, it was aimed to determine essential oil and extracts, which are known as Ferula communis and obtained from naturally grown giant fennel plants in Agn province, antioxidant effects and antimicrobial effects against Chryseobacterium indologenes which cause soft rot in certain vegetables and fruits in vitro. Also was determined the chemical composition of essential oils used in this study. For this purpose, 8 C. indologenes isolates, which were determined to be soft decay agents in cucumber, tomato, pepper and squash, were used in previous studies. Laboratory trials were established in triplicate and penicillin and kanamycin antibiotics, which are commercially available, were used as positive controls in the experiments. According to the obtained results; It has been observed that essential oils gained from F. communis plant have bactericidal activity against bacterial pathogens in the ratio of 12-21 mm. However, none of the extracts obtained from the F. communis plant used in the study were found to have antibacterial activity against C. indolenes species. Antibiotics used as positive controls have also been observed to have no effect against pathogens as in the extracts. As a result, it has been determined that the essential oil used in the study has a lethal effect against 8 different C. indologenes which have soft caries pathogens, but the extracts and antibiotics used have no antimicrobial effect. In addition, the chemical composition of essential oil determined to be effective in the study has been determined. For this reason, it is considered that the essential oil obtained from F. communis plant can be used successfully in the control against C. indologenes pathogens. This result shows how much the F. communis essential oil has a great importance in control against these pathogens very resistant to chemical drugs. In this study, antioxidant capacity of F. communis methanol, acetone, chloroform, hexane extract and essential oil was determined by DPPH and ABTS method, phenolic substance contents and folin was determined by cycaltio method. Water extract had highest ABTS free radical scavenging activity with 88.07% (0.05 mg / ml), (DPPH) capacity with 52,04% (50 mu g/ml) and highest amound of phenolic compound with 13,25 (mg GAE/gr).