PCR amplification of SNP loci from crude DNA for large-scale genotyping of oomycetes

被引：2
|
作者：
Hu, Jian [1 ,2 ]
Lyon, Rebecca [2 ]
Zhou, Yuxin [2 ]
Lamour, Kurt [2 ]
机构：
[1] China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China
[2] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA
来源：
MYCOLOGIA | 2014年 / 106卷 / 03期
关键词：
Peronospora; Phytophthora; SNP genotyping;
D O I：
10.3852/13-218
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Similar to other eukaryotes, single nucleotide polymorphism (SNP) markers are abundant in many oomycete plant pathogen genomes. High resolution DNA melting analysis (HR-DMA) is a cost-effective method for SNP genotyping, but like many SNP marker technologies, is limited by the amount and quality of template DNA. We describe PCR preamplification of Phytophthora and Peronospora SNP loci from crude DNA extracted from a small amount of mycelium and/or infected plant tissue to produce sufficient template to genotype at least 10 000 SNPs. The approach is fast, inexpensive, requires minimal biological material and should be useful for many organisms in a variety of contexts.
引用
收藏
页码：607 / 609
页数：3
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