Circulating cell-free DNA in patients with alveolar echinococcosis

被引：20

作者：

Baraquin, Alice ^{[1
]}

Hervouet, Eric ^{[2
,3
]}

Richou, Carine ^{[4
]}

Flori, Pierre ^{[5
,6
]}

Peixoto, Paul ^{[2
,3
]}

Azizi, Amel ^{[7
]}

Delabrousse, Eric ^{[7
]}

Blagosklonov, Oleg ^{[8
]}

Umhang, Gerald ^{[9
]}

Bresson-Hadni, Solange ^{[10
]}

Valot, Benoit ^{[1
]}

Grenouillet, Frederic ^{[1
,11
]}

Felix, Sophie ^{[12
]}

Heyd, Bruno ^{[12
]}

Mantion, Georges ^{[12
]}

Di Martino, Vincent ^{[12
]}

Montange, Damien ^{[12
]}

Vanlemmens, Claire ^{[12
]}

Vuitton, Dominique Angele ^{[12
]}

Weil-Verhoeven, Delphine ^{[12
]}

Chavanet, Pascal ^{[13
]}

Dalle, Frederic ^{[13
]}

Gohier, Sandrine ^{[13
]}

Minello, Anne ^{[13
]}

Piroth, Lionel ^{[13
]}

Dumortier, Jerome ^{[14
]}

Mabrut, Jean-Yves ^{[14
]}

Wallon, Martine ^{[14
]}

Frentiu, Emilia ^{[15
]}

Machouart, Marie ^{[15
]}

Watelet, Jerome ^{[15
]}

Chemla, Cathy ^{[16
]}

Feron, Thomas ^{[16
]}

Heurge-Berlot, Alexandra ^{[16
]}

Sommacale, Daniele ^{[16
]}

Thiefin, Gerard ^{[16
]}

Abou-Bacar, Ahmed ^{[17
]}

Brunet, Julie ^{[17
]}

Candolfi, Erman ^{[17
]}

Hansmann, Yves ^{[17
]}

Lefebvre, Nicolas ^{[17
]}

机构：

[1] Univ Bourgogne Franche Comte, CNRS, UMR 6249, Chronoenvironnem,UBFC,Aff INRA, Besancon, France

[2] Univ Bourgogne Franche Comte, UMR 1098, INSERM,EFS BFC,UBFC, Interact Hote Greffon Tumeur Ingn Cellulaire & Ge, Besancon, France

[3] EPIGENExp EPIgenet & GENe EXPress Tech Platform, Besancon, France

[4] Univ Hosp, Hepatol Dept, Besancon, France

[5] Fac Med Jacques Lisfranc, GIMAP Grp Immun Muqueuses & Agents Pathogenes, EA 3064, St Etienne, France

[6] Univ Hosp, Pole Biol Pathol Parasitol & Mycol Lab, St Etienne, France

[7] Univ Hosp, Radiol Dept, Besancon, France

[8] Univ Hosp, Dept Nucl Med, Besancon, France

[9] ANSES LRFSN, Natl Reference Lab Echinococcus Spp, Wildlife Surveillance & Ecoepidemiol Unit, Malzeville, France

[10] Univ Hosp, Visceral Digest & Oncol Dept, Besancon, France

[11] Univ Hosp, Dept Parasit & Fungal Serol, Besancon, France

[12] Univ Hosp Besancon, Besancon, France

[13] Univ Hosp Dijon, Dijon, France

[14] HCL Lyon, Lyon, France

[15] Univ Hosp Nancy, Nancy, France

[16] Univ Hosp Reims, Reims, France

[17] Univ Hosp Strasbourg, Strasbourg, France

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2018年 / 222卷

关键词：

Alveolar echinococcosis; Circulating cell-free DNA; Digital PCR; Biomarker; DROPLET DIGITAL PCR; TUMOR DNA; COPY NUMBER; CANCER; MULTILOCULARIS; EFFICACY; QUANTIFICATION; RESPONSES; VESICLES; BIOPSY;

D O I：

10.1016/j.molbiopara.2018.04.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alveolar echinococcosis (AE) is a parasitic disease, due to Echinococcus multilocularis. Often compared to liver cancer, it develops by infiltration from its primary site to the surrounding tissue, and can then metastasize to other organs. Detection of circulating cell-free DNA (ccfDNA) is a useful analytical tool in oncology, for diagnosis, prognosis, and therapy monitoring. This study sought to investigate the presence of ccfDNA in patients with AE, and its potential usefulness for the evaluation of treatment efficiency. To achieve these aims, a quantitative PCR and a droplet digital PCR were developed to detect E. multilocularis ccfDNA. An AE animal model identified, for the first time, the presence of large quantities of ccfDNA. Samples from patients with AE (n = 31) were then analyzed twice, at diagnosis, and after three months of chemotherapy: about 25% were positive, almost always with very low concentrations of ccfDNA. These results confirmed that E. multilocularis produces ccfDNA, as solid tumors do, but detection may not yet be sufficient for AE diagnosis nor for the evaluation of treatment efficiency, due to the low levels of ccfDNA detected in patient serum.

引用

页码：14 / 20

页数：7

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