Lateral displacement as a function of particle size using a piecewise curved planar interdigitated electrode array

被引:51
|
作者
Han, Ki-Ho [1 ]
Han, Song-I [1 ]
Frazier, A. Bruno [2 ]
机构
[1] Inje Univ, Sch Nano Engn, Gimhae 621749, GyongNam, South Korea
[2] Georgia Inst Technol, Sch Elect & Comp Engn, Atlanta, GA 30332 USA
关键词
BREAST-CANCER CELLS; FIELD-FLOW-FRACTIONATION; DIELECTROPHORETIC SEPARATION; PERIPHERAL-BLOOD; TUMOR-CELLS; STEM-CELLS; ENRICHMENT; ELECTROROTATION; MICRODEVICES; MANIPULATION;
D O I
10.1039/b909753h
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe the lateral displacement of a particle passing over a planar interdigitated electrode array at an angle as a function of the particle size. The lateral displacement was also measured as a function of the angle between the electrode and the direction of flow. A simplified line charge model was used for numerically estimating the lateral displacement of fluorescent polystyrene (PS) beads with three different diameters. Using the lateral displacement as a function of particle size, we developed a lateral dielectrophoretic (DEP) microseparator, which enables continuous discrimination of particles by size. The microchannel was divided into three regions, each with an electrode array placed at a different angle with respect to the direction of flow. The experiment using an admixture of 3-, 5-, and 10-mu m PS beads showed that the lateral DEP microseparator could continuously separate out 99.86% of the 3-mu m beads, 98.82% of the 5-mu m beads, and 99.69% of the 10-mu m beads, simply by using a 200-kHz 12-Vp-p AC voltage to create the lateral DEP force. The lateral DEP microseparator is thus a practical device for simultaneously separating particles according to size from a heterogeneous admixture.
引用
收藏
页码:2958 / 2964
页数:7
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