Understanding Life at High Temperatures: Relationships of Molecular Channels in Enzymes of Methanogenic Archaea and Their Growth Temperatures

被引:0
|
作者
Ginsbach, Laura F. [1 ]
Gonzalez, Juan M. [2 ]
机构
[1] Tech Univ Berlin, Inst Biotechnol, Dept Appl Biochem, D-10623 Berlin, Germany
[2] CSIC, Inst Recursos Nat & Agrobiol Sevilla, IRNAS CSIC, Seville 41012, Spain
关键词
molecular channels; molecular tunnels; hyperthermophiles; thermal stability; methanogen; Archaea; GLUTAMATE-DEHYDROGENASE; STABILITY; TUNNELS;
D O I
10.3390/ijms232315149
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Analyses of protein structures have shown the existence of molecular channels in enzymes from Prokaryotes. Those molecular channels suggest a critical role of spatial voids in proteins, above all, in those enzymes functioning under high temperature. It is expected that these spaces within the protein structure are required to access the active site and to maximize availability and thermal stability of their substrates and cofactors. Interestingly, numerous substrates and cofactors have been reported to be highly temperature-sensitive biomolecules. Methanogens represent a singular phylogenetic group of Archaea that performs anaerobic respiration producing methane during growth. Methanogens inhabit a variety of environments including the full range of temperatures for the known living forms. Herein, we carry out a dimensional analysis of molecular tunnels in key enzymes of the methanogenic pathway from methanogenic Archaea growing optimally over a broad temperature range. We aim to determine whether the dimensions of the molecular tunnels are critical for those enzymes from thermophiles. Results showed that at increasing growth temperature the dimensions of molecular tunnels in the enzymes methyl-coenzyme M reductase and heterodisulfide reductase become increasingly restrictive and present strict limits at the highest growth temperatures, i.e., for hyperthermophilic methanogens. However, growth at lower temperature allows a wide dimensional range for the molecular spaces in these enzymes. This is in agreement with previous suggestions on a potential major role of molecular tunnels to maintain biomolecule stability and activity of some enzymes in microorganisms growing at high temperatures. These results contribute to better understand archaeal growth at high temperatures. Furthermore, an optimization of the dimensions of molecular tunnels would represent an important adaptation required to maintain the activity of key enzymes of the methanogenic pathway for those methanogens growing optimally at high temperatures.
引用
收藏
页数:9
相关论文
共 50 条
  • [21] The stability of biomolecules and the implications for life at high temperatures
    Daniel, RM
    van Eckert, R
    Holden, JF
    Truter, J
    Cowan, DA
    SUBSEAFLOOR BIOSPHERE AT MID-OCEAN RANGES, 2004, 144 : 25 - 39
  • [22] Towards a better understanding of superconductivity at high transition temperatures
    R. Hackl
    W. Hanke
    The European Physical Journal Special Topics, 2010, 188 : 3 - 14
  • [23] Towards a better understanding of superconductivity at high transition temperatures
    Hackl, R.
    Hanke, W.
    EUROPEAN PHYSICAL JOURNAL-SPECIAL TOPICS, 2010, 188 (01): : 3 - 14
  • [24] From Water Under Confinement to Understanding Life at Subzero Temperatures
    Yao, Yang
    CHIMIA, 2024, 78 (10) : 659 - 664
  • [25] MOLECULAR BASIS OF BIOLOGICAL STABILITY TO HIGH TEMPERATURES
    KOFFLER, H
    MALLETT, GE
    ADYE, J
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1957, 43 (06) : 464 - 477
  • [26] Effect of molecular nitrogen on molybdenum at high temperatures
    Tury, P
    Krausz, S
    NATURE, 1936, 138 : 331 - 331
  • [27] Molecular dynamics simulations of graphite at high temperatures
    Hehr, Brian D.
    Hawari, Ayman I.
    Gillette, Victor H.
    NUCLEAR TECHNOLOGY, 2007, 160 (02) : 251 - 256
  • [28] MOLECULAR ABSORPTION OF SOUND IN GASES AT HIGH TEMPERATURES
    YOUNG, JE
    MAWARDI, OK
    JOURNAL OF CHEMICAL PHYSICS, 1956, 24 (05): : 1109 - 1109
  • [29] Molecular simulations suggest protein salt bridges are uniquely suited to life at high temperatures
    Thomas, AS
    Elcock, AH
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (07) : 2208 - 2214
  • [30] Survivability and growth kinetics of methanogenic archaea at various pHs and pressures: Implications for deep subsurface life on Mars
    Sinha, Navita
    Nepal, Sudip
    Kral, Timothy
    Kumar, Pradeep
    PLANETARY AND SPACE SCIENCE, 2017, 136 : 15 - 24