Microplate fluorescence assay for the quantification of double stranded DNA using SYBR Green I dye

被引：56

作者：

Leggate, Johanna ^{[1
]}

Allain, Ray ^{[1
]}

Isaac, Leah ^{[1
]}

Blais, Burton W. ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Ottawa Lab Carling, CEF, Ottawa, ON K1A 0C6, Canada

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 19期

关键词：

DNA quantification; microplate fluorescence assay; SYBR Green I;

D O I：

10.1007/s10529-006-9128-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A high-throughput, 96-well microplate fluorescence assay (MFA) was developed for DNA quantification using the double-stranded DNA-binding dye SYBR Green I. Samples mixed with SYBR Green I in the wells of a microtiter plate produced fluorescence in proportion with DNA concentration which was measured using a fluorescence plate reader. The performance characteristics of the assay were compared with spectrophotometric quantification based on ultraviolet absorption and the Hoefer DyNA Quant assay utilizing the fluorescent dye, Hoechst 33258. The MFA accurately quantified different types of DNA over a broad linear dynamic range of concentrations (0.25-2,500 pg/mu l), and was not affected by a variety of contaminants in the assay mixture.

引用

页码：1587 / 1594

页数：8

共 50 条

[21] Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen
Sang, Fuming
Ren, Jicun
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (09) : 1275 - 1280
[22] CGE-laser induced fluorescence of double-stranded DNA fragments using GelGreen dye
Valdes, Alberto
Garcia-Canas, Virginia
Cifuentes, Alejandro
ELECTROPHORESIS, 2013, 34 (11) : 1555 - 1562
[23] Photocatalytic oxidation of TMB with the double stranded DNA-SYBR Green I complex for label-free and universal colorimetric bioassay
Zhang, Xinfeng
Huang, Chengpeng
Xu, Shuxia
Chen, Junbo
Zeng, Ying
Wu, Peng
Hou, Xiandeng
CHEMICAL COMMUNICATIONS, 2015, 51 (77) : 14465 - 14468
[24] A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I
Zheng, Aihua
Luo, Ming
Xiang, Dongshan
Xiang, Xia
Ji, Xinghu
He, Zhike
TALANTA, 2013, 114 : 49 - 53
[25] Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR green I fluorescence
Yasuda, T
Takeshita, H
Nakazato, E
Nakajima, T
Hosomi, O
Nakashima, Y
Kishi, K
ANALYTICAL BIOCHEMISTRY, 1998, 255 (02) : 274 - 276
[26] Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR green I fluorescence
Department of Legal Medicine, Gunma University, School of Medicine, Maebashi, Gunma 371, Japan
Anal. Biochem., 2 (274-276):
[27] Visualization of DNA-containing structures in various species ofChlorophyta, Rhodophyta andCyanophyta using SYBR green I dye
M. Vítová
J. Hendrychová
V. Cepák
V. Zachleder
Folia Microbiologica, 2005, 50 : 333 - 340
[28] Visualization of DNA-containing structures in various species of Chlorophyta, Rhodophyta and Cyanophyta using SYBR Green I dye
Vítová, M
Hendrychová, J
Cepák, V
Zachleder, V
FOLIA MICROBIOLOGICA, 2005, 50 (04) : 333 - 340
[29] Metal-enhanced PicoGreen fluorescence: Application for double-stranded DNA quantification
Dragan, A. I.
Bishop, E. S.
Casas-Finet, J. R.
Strouse, R. J.
Schenerman, M. A.
Geddes, C. D.
ANALYTICAL BIOCHEMISTRY, 2010, 396 (01) : 8 - 12
[30] Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide
Xia, Jiaoyun
Xu, Tong
Qing, Jing
Wang, Lihua
Tang, Junlong
FRONTIERS IN CHEMISTRY, 2021, 9

← 1 2 3 4 5 →