DNA sequencing by capillary array electrophoresis with an electric field strength gradient

被引：16
|
作者：
Endo, Y [1 ]
Yoshida, C [1 ]
Baba, Y [1 ]
机构：
[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Med Chem, Tokushima 7708505, Japan
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1999年 / 41卷 / 2-3期
关键词：
DNA sequencing; dye terminator; gradient of electric field strength;
D O I：
10.1016/S0165-022X(99)00043-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We examined the effect of an electric field strength gradient on DNA sequencing efficiency using capillary array electrophoresis. Several types of gradients were applied to DNA sequencing and tested in terms of read length and accuracy. Our original method improved the accuracy of DNA sequencing for longer fragments at high temperature. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：133 / 141
页数：9
相关论文
共 50 条
  • [1] Enhanced throughput for DNA sequencing by capillary array electrophoresis with a gradient of electric field strength
    Yoshida, C
    Endo, Y
    Baba, Y
    [J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2001, 13 (01) : 99 - 103
  • [2] Enhanced separation of DNA sequencing products by capillary electrophoresis using a stepwise gradient of electric field strength
    Inoue, H
    Tsuhako, M
    Baba, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 802 (01) : 179 - 184
  • [3] OPTIMIZATION OF ELECTRIC-FIELD STRENGTH FOR DNA-SEQUENCING IN CAPILLARY GEL-ELECTROPHORESIS
    LUCKEY, JA
    SMITH, LM
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (20) : 2841 - 2850
  • [4] Capillary array electrophoresis DNA sequencing.
    Kheterpal, I
    Mathies, RA
    [J]. ANALYTICAL CHEMISTRY, 1999, 71 (01) : 31A - 37A
  • [5] DNA SEQUENCING USING CAPILLARY ARRAY ELECTROPHORESIS
    HUANG, XHC
    QUESADA, MA
    MATHIES, RA
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (18) : 2149 - 2154
  • [6] DNA sequencing by capillary array electrophoresis and microfabricated array systems
    Carrilho, E
    [J]. ELECTROPHORESIS, 2000, 21 (01) : 55 - 65
  • [7] Sub-microliter DNA sequencing for capillary array electrophoresis
    Hadd, AG
    Goard, MP
    Rank, DR
    Jovanovich, SB
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 894 (1-2) : 191 - 201
  • [8] DNA sequencing using 96-capillary array electrophoresis
    Pang, HM
    Pavski, V
    Yeung, ES
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1999, 41 (2-3): : 121 - 132
  • [9] DNA sequencing by capillary electrophoresis
    Dovichi, NJ
    [J]. ELECTROPHORESIS, 1997, 18 (12-13) : 2393 - 2399
  • [10] High-throughput DNA sequencing on a capillary array electrophoresis system
    Marsh, M
    Tu, O
    Dolnik, V
    Roach, D
    Solomon, N
    Bechtol, K
    Smietana, P
    Wang, LP
    Li, XD
    Cartwright, P
    Marks, A
    Barker, D
    Harris, D
    Bashkin, J
    [J]. JOURNAL OF CAPILLARY ELECTROPHORESIS, 1997, 4 (02) : 83 - 89
12345