Fluorescence lifetime imaging using a compact, low-cost, diode-based all-solid-state regenerative amplifier

被引：4
|
作者：
Mendez, E [1 ]
Elson, DS [1 ]
Koeberg, M [1 ]
Dunsby, C [1 ]
Bradley, DDC [1 ]
French, PMW [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Blackett Lab, London SW7 2BW, England
来源：
REVIEW OF SCIENTIFIC INSTRUMENTS | 2004年 / 75卷 / 05期
关键词：
D O I：
10.1063/1.1710701
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A fluorescence lifetime imaging (FLIM) system is described that utilizes a new compact and low-cost ultrafast laser source based on a gain-switched laser diode-seeded all-solid-state Cr:LiSAF regenerative amplifier that has been designed for this application. The pulse parameters of this source (0.5 muJ, 827 nm, 100 ps, 5 kHz) are shown to be appropriate to time-domain FLIM using a gated optical intensifier and the application to functional imaging of biological tissue is demonstrated, as well as the first evaluation of organic light emitting diodes using FLIM. (C) 2004 American Institute of Physics.
引用
收藏
页码：1264 / 1267
页数：4
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