Isolation of Murine Primary Aortic Smooth Muscle Cells

被引：10

作者：

Hubert, Max Ole ^{[1
]}

Rodriguez-Vita, Juan ^{[1
]}

Wiedmann, Lena ^{[1
]}

Fischer, Andreas ^{[1
,2
,3
]}

机构：

[1] German Canc Res Ctr, Div Vasc Signaling & Canc, Heidelberg, Germany

[2] Heidelberg Univ, Med Fac Mannheim, European Ctr Angiosci, Mannheim, Germany

[3] Heidelberg Univ Hosp, Endocrinol & Clin Chem, Med Clin 1, Heidelberg, Germany

来源：

BIO-PROTOCOL | 2021年 / 11卷 / 03期

关键词：

Vascular smooth muscle cells; Murine aorta; Extraction; Isolation; Cultivation; GROWTH-FACTOR-BETA;

D O I：

10.21769/BioProtoc.3907

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. The most common source of VSMCs is the rat aorta. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The advantage of this method is that there are many more transgenic mouse lines available compared to rats. The protocol consists of the isolation of the aorta, the liberation of vascular cells by the action of collagenase, culturing of VSCMs, and analyzing filamentous actin and alpha smooth muscle actin by fluorescence microscopy. VSCMs can be further used to study mechanisms underlying cardiovascular diseases. [GRAPHICS] .

引用

页数：10

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