In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats

被引：0
|
作者：
Jaroenporn, Chanokchon [1 ]
Supawasit, Wannakarn [1 ]
Bundidamorn, Damkerng [1 ,2 ]
Udompijitkul, Pathima [1 ,2 ]
Assawamakin, Anunchai [3 ]
Trevanich, Sudsai [1 ,2 ]
机构：
[1] Kasetsart Univ, Fac Agroind, Dept Food Sci & Technol, Bangkok 10900, Thailand
[2] Kasetsart Univ, Ctr Adv Studies Agr & Food, Inst Adv Studies, Bangkok 10900, Thailand
[3] Mahidol Univ, Fac Pharm, Dept Pharmacol, Bangkok 10400, Thailand
来源：
FOODS | 2022年 / 11卷 / 11期
关键词：
Multiplex PCR; Shiga toxin-producing Escherichia coli; Listeria monocytogenes; Salmonella spp; validation; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; TYPHIMURIUM; ENTERICA; ENTERITIDIS; PREVALENCE; O157H7; CATTLE; ASSAY; FOOD;
D O I：
10.3390/foods11111557
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of the study was to perform in-house validation of the developed multiplex PCR (mPCR)-based alternative method to detect Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw meats following the ISO 16140-2: 2016. A comparative study of the developed mPCR against the Bacteriological Analytical Manual (BAM) method was evaluated for inclusivity and exclusivity, sensitivity and the relative level of detection (RLOD). Inclusivity levels for each target bacterium were all 100%, while exclusivity for non-target bacteria was 100%. The sensitivity of the developed mPCR was calculated based on the analysis of 72 samples of raw meat. The sensitivity of the developed mPCR was 100%. The RLOD values of the developed mPCR for STEC, L. monocytogenes and Salmonella spp. were 0.756, 1.170 and 1.000, respectively. The developed mPCR showed potential as a tool for the fast, specific and sensitive detection of the three bacteria in the raw meat industry
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页数：12
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