Pseudomonas sauna sp nov., isolated from a salt lake

A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85(T), was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85T were non-endospore-forming rods, 0.4-0.6 mu m wide and 1.0-1.6 gm long, and motile by means of a single polar flagellum. Strain XCD-X85T was catalase- and oxidase-positive. Growth was observed in the presence of 0-12.0 % (w/v) NaCI (optimum, 1.0-2.0 %) and at 4-35 degrees C (optimum, 25-30 degrees C) and pH 6.5-10.5 (optimum, pH 8.0-8.5). Strain XCD-X85T contained (>10 %) summed feature 8 (C-18 : 1 omega 7c and/or C-18 (:1)omega 6c, C-12: 0, C-16 :0 and summed feature 3 (C-16 :1 omega 7c and/or C-16 : (1)omega 6c) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (0-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G + C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85(T) was associated with the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas pelagia CL-AP6(T) (99.0 %) and Pseudomonas bauzanensis BZ93(T) (96.8 %). DNA-DNA relatedness of strain XCD-X85(T) to P. pelagia JCM 15562(T) was 19 +/- %. On the basis of the data presented above, it is concluded that strain XCD-X85(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salina sp. nov. is proposed. The type strain is XCD-X85(T) (=CGMCC 1.12482(T)=JCM 19469(T)).