A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons

被引：4
|
作者：
Zhao, B. [1 ]
Wang, H. [1 ]
Mao, X. [1 ]
Li, R. [1 ]
机构：
[1] Tsinghua Univ, Dept Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 49卷 / 03期
基金：
中国国家自然科学基金;
关键词：
cellulose acetate; nitrate filter; PAH-degrading bacteria; polycyclic aromatic hydrocarbons; screening method; ENUMERATION; METABOLISM; SITES;
D O I：
10.1111/j.1472-765X.2009.02668.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: A rapid procedure was developed to screen for bacteria that are able to grow on polycyclic aromatic hydrocarbons (PAHs). Methods and Results: A drop of ethyl ether-dissolved PAH is spread on a sterilized cellulose acetate/nitrate filter lying on the top of a mineral salts agarose plate. After the evaporation of ethyl ether, a serially diluted sample is spread over the filter and incubated. Subsequently, the PAH degrading bacteria can be counted and isolated. Conclusions: This procedure is a simple method for screening bacterial isolates for the ability to grow with PAHs. Significance and Impact of the Study: This technique is rapid to screen and/or count PAH-degrading bacteria and is also used to streak cultivation without disrupting the PAH layer on plate.
引用
收藏
页码：408 / 410
页数：3
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