Safety assessment of Cry1Ab/Ac fusion protein

被引：58

作者：

Xu, Wentao ^{[1
,2
]}

Cao, Sishuo ^{[1
]}

He, Xiaoyun ^{[1
]}

Luo, YunBo ^{[1
]}

Guo, Xing ^{[1
]}

Yuan, Yanfang ^{[1
]}

Huang, Kunlun ^{[1
,2
]}

机构：

[1] China Agr Univ, Lab Food Safety & Mol Biol, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[2] Minist Agr, Supervis Inspect & Testing Ctr Genetically Modifi, Beijing 100083, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2009年 / 47卷 / 07期

关键词：

Allergenicity; Cry1Ab/Ac fusion protein; Oral acute study; Safety assessment; GMO; BACILLUS-THURINGIENSIS TOXIN; TRANSGENIC PLANTS; COTTONSEED MEAL; FOOD ALLERGENS; WISTAR RATS; IN-VITRO; TOXICITY; PROTOXIN; MICE; BIOTECHNOLOGY;

D O I：

10.1016/j.fct.2009.03.029

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Cry1ab/ac gene was fused by both the cry1ab gene (GenBank Accession No. X54939) and the cry1ac gene (GenBank Accession No. Y09787), which was widely used in genetically modified (GM) rice, cotton, maize and so on. In order to support the safety assessment of CM food or feed products containing Cry1Ab/Ac protein, sufficient quantities of Cry1Ab/Ac protein were produced in Escherichia coli for in vitro evaluation and animal studies. The Cry1Ab/Ac protein does not possess the characteristics associated with food toxins or allergens, i.e., it has no sequence homology with any known allergens or toxins, and no N-glycosylation sites, can be rapidly degraded in gastric and intestinal fluids, and is devoid of adverse effects in mice by gavage at a high dose level of 5 g (Cry1Ab/Ac protein)/kg body weight. In conclusion, there is a reasonable certainty of no harm resulting from the inclusion of the Cry1Ab/Ac protein in human food or animal feed. (C) 2009 Elsevier Ltd. All rights reserved

引用

页码：1459 / 1465

页数：7

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