Inhibition of γ-radiation induced DNA damage in plasmid pBR322 by TMG, a water-soluble derivative of vitamin E

被引：42
|
作者：
Rajagopalan, R
Wani, K
Huilgol, NG
Kagiya, TV
Nair, CKK [1 ]
机构：
[1] Bhabha Atom Res Ctr, Div Radiat Biol, Bombay 400085, Maharashtra, India
[2] Nanavati Hosp & MRC, Dept Radiat Oncol, Vile Parle, India
[3] Kinki Res Fdn, Kyoto, Japan
来源：
JOURNAL OF RADIATION RESEARCH | 2002年 / 43卷 / 02期
关键词：
DNA strandbreaks; thymine glycol; radiation protection; tocopherol monoglucoside;
D O I：
10.1269/jrr.43.153
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Alpha-tocopherol monoglucoside (TMG), a water-soluble derivative of alpha-tocopherol, has been examined for its ability to protect DNA against radiation-induced strand breaks. Gamma radiation, up to a dose of 6 Gy (dose rate, 0.7 Gy/ minute), induced a dose-dependent increase in single strand breaks (SSBs) in plasmid pBR322 DNA. TMG inhibited the formation of gamma-radiation induced DNA single strand breaks (SSBs) in a concentration-dependent manner; 500 muM of TMG protected the single strand breaks completely. It also protected thymine glycol formation induced by gamma-radiation in a dose-dependent manner, based on an estimation of thymine glycol by HPLC.
引用
收藏
页码：153 / 159
页数：7
相关论文
共 50 条
  • [1] Inhibition of radiation-induced DNA damage in plasmid pBR322 by chlorophyllin and possible mechanism(s) of action
    Kumar, SS
    Chaubey, RC
    Devasagayam, TPA
    Priyadarsini, KI
    Chauhan, PS
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1999, 425 (01) : 71 - 79
  • [2] Mechanism of protection against radiation-induced DNA damage in plasmid pBR322 by caffeine
    Kumar, SS
    Devasagayam, TPA
    Jayashree, B
    Kesavan, PC
    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2001, 77 (05) : 617 - 623
  • [3] E-coli growth inhibition by a high copy number derivative of plasmid pBR322
    Valenzuela, MS
    Ikpeazu, EV
    Siddiqui, KAI
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 219 (03) : 876 - 883
  • [4] OXYGEN FREE-RADICAL MEDIATED DAMAGE TO PBR322 PLASMID DNA
    SCHNEIDER, JE
    BROWNING, MM
    FLOYD, RA
    FASEB JOURNAL, 1988, 2 (06): : A1762 - A1762
  • [5] OXYGEN FREE-RADICAL MEDIATED DAMAGE TO PBR322 PLASMID DNA
    SCHNEIDER, JE
    BROWNING, MM
    FLOYD, RA
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1988, 29 : 110 - 110
  • [6] Damage to pBR322 DNA photosensitized by hypocrellin A in liposomes and its derivative in solution
    Zou, W
    An, JY
    Jiang, LJ
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1996, 33 (01) : 73 - 78
  • [7] In vitro DNA damage characterisation studies on plasmid pBR322 after exposure to γ radiation by 60Co
    Vedansha Jaiswal
    Pragati Misra
    P. K. Shukla
    P. W. Ramteke
    A. B. Tiku
    Journal of Radioanalytical and Nuclear Chemistry, 2012, 291 : 661 - 664
  • [8] In vitro DNA damage characterisation studies on plasmid pBR322 after exposure to γ radiation by 60Co
    Jaiswal, Vedansha
    Misra, Pragati
    Shukla, P. K.
    Ramteke, P. W.
    Tiku, A. B.
    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 2012, 291 (03) : 661 - 664
  • [9] ASCORBATE IRON MEDIATION OF HYDROXYL FREE-RADICAL DAMAGE TO PBR322 PLASMID DNA
    SCHNEIDER, JE
    BROWNING, MM
    FLOYD, RA
    FREE RADICAL BIOLOGY AND MEDICINE, 1988, 5 (5-6) : 287 - 295
  • [10] BIOLOGICAL INACTIVATION OF PBR322 PLASMID DNA BY ENZYME-INDUCED AND RADIATION-INDUCED SINGLE-STRAND DAMAGE UNDER VARIOUS CONDITIONS
    VENTUR, Y
    SCHULTEFROHLINDE, D
    MUTATION RESEARCH, 1994, 315 (01): : 65 - 74
12345