Ursolic acid modulates MMPs, collagen-I, α-SMA, and TGF-β expression in isoproterenol-induced myocardial infarction in rats

被引:34
|
作者
Radhiga, T. [1 ]
Senthil, S. [1 ]
Sundaresan, A. [1 ]
Pugalendi, K. V. [1 ]
机构
[1] Annamalai Univ, Dept Biochem & Biotechnol, Fac Sci, Chidambaram, Tamil Nadu, India
关键词
Mitochondrial enzymes; lysosomal enzymes; cardiac fibrosis; triterpenoid; catecholamine; MATRIX METALLOPROTEINASES; COLORIMETRIC METHOD; TISSUE INHIBITORS; LYSOSOMAL-ENZYMES; CYTOCHROME-C; FIBROSIS; MITOCHONDRIAL; TRANSPORT; APOPTOSIS; DAMAGE;
D O I
10.1177/0960327119842620
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
In the present study, the modulatory effect of ursolic acid (UA) on cardiac fibrosis and mitochondrial and lysosomal enzymes activity in isoproterenol-induced myocardial infarction (MI) in rats were examined. Isoproterenol hydrochloride (ISO; 85 mg/kg body weight) was administered subcutaneously for first two consecutive days. ISO-induced MI in rats significantly decreased the activities of mitochondrial tricarboxylic acid cycle enzymes and respiratory chain enzymes while increased the activities of lysosomal glycohydrolases and cathepsins. The expression of matrix metalloproteinase 2 (MMP-2), MMP-9, collagen type I, alpha-smooth muscle actin (alpha-SMA), and transforming growth factor-beta (TGF-beta) were upregulated in ISO-induced MI in rats. UA administration to rats showed increased activities of mitochondrial tricarboxylic acid cycle enzymes and respiratory chain enzymes and decreased activities of lysosomal glycohydrolases and cathepsins in ISO-induced rats. Furthermore, expression of MMP-2, MMP-9, collagen type I, alpha-SMA, and TGF-beta downregulated in UA-administered rats. Thus, our results demonstrate that UA has an anti-fibrotic effect and attenuates the mitochondrial and lysosomal dysfunction in ISO-induced MI in rats.
引用
收藏
页码:785 / 793
页数:9
相关论文
共 50 条
  • [31] Effects of ursolic acid on expression of TGFβ1 and α-SMA in rats with liver fibrosis
    Ou-Yang, C-H
    Zhu, X.
    Zhang, K-H
    Dai, Y.
    Chen, J.
    He, W-H
    Li, B.
    Li, B-M
    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2009, 24 : A146 - A146
  • [32] Effect of Fraxetin on Oxidative Damage Caused by Isoproterenol-Induced Myocardial Infarction in Rats
    Yu Yin
    Lihui Wang
    Guifang Chen
    Hongwen You
    Applied Biochemistry and Biotechnology, 2022, 194 : 5666 - 5679
  • [33] ISOPROTERENOL-INDUCED MYOCARDIAL-INFARCTION IN RATS - FUNCTIONAL AND BIOCHEMICAL-ALTERATIONS
    NIRMALA, C
    PUVANAKRISHNAN, R
    MEDICAL SCIENCE RESEARCH, 1994, 22 (08): : 575 - 577
  • [34] Effects of low dose of aliskiren on isoproterenol-induced acute myocardial infarction in rats
    Bin-Jaliah, I
    Hussein, A. M.
    Sakr, H. F.
    Eid, E. A.
    PHYSIOLOGY INTERNATIONAL, 2018, 105 (02) : 127 - 144
  • [35] The effect of L-lysine on serum sialic acid levels in isoproterenol-induced myocardial infarction in rats
    Uzgur, Selda
    Usta, Ufuk
    Goekmen, Selma Sueer
    TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, 2011, 36 (03): : 248 - 254
  • [36] Cardioprotective effect of rosuvastatin against isoproterenol-induced myocardial infarction injury in rats
    Yu, Ying
    Jin, Lin
    Zhuang, Yamin
    Hu, Yan
    Cang, Jing
    Guo, Kefang
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018, 41 (06) : 3509 - 3516
  • [37] Effect of Fraxetin on Oxidative Damage Caused by Isoproterenol-Induced Myocardial Infarction in Rats
    Yin, Yu
    Wang, Lihui
    Chen, Guifang
    You, Hongwen
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2022, 194 (12) : 5666 - 5679
  • [38] SERUM PROLACTIN LEVELS IN RATS FOLLOWING ISOPROTERENOL-INDUCED MYOCARDIAL-INFARCTION
    LEWIS, BK
    WEXLER, BC
    PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1975, 150 (03): : 712 - 717
  • [39] Ameliorative Effects of Ponicidin Against the Isoproterenol-induced Acute Myocardial Infarction in Rats
    Liu, Manli
    Seshadri, Vidya Devanathadesikan
    Jiang, Qihua
    PHARMACOGNOSY MAGAZINE, 2023, 19 (02) : 427 - 438
  • [40] INFLUENCE OF ISOPROTERENOL-INDUCED MYOCARDIAL-INFARCTION ON CERTAIN GLYCOHYDROLASES AND CATHEPSINS IN RATS
    RAVICHANDRAN, LV
    PUVANAKRISHNAN, R
    JOSEPH, KT
    BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, 1991, 45 (01): : 6 - 15