Differential Effects of Ceramide on Cell Viability and Extracellular Matrix Remodeling in Keratinocytes and Fibroblasts

被引：15
|
作者：
Philips, N. [1 ]
Tuason, M. [1 ]
Chang, T. [2 ]
Lin, Y. [1 ]
Tahir, M. [1 ]
Rodriguez, S. G. [3 ]
机构：
[1] Fairleigh Dickinson Univ, Teaneck, NJ 07666 USA
[2] Bergen Cty Acad, Hackensack, NJ USA
[3] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
来源：
SKIN PHARMACOLOGY AND PHYSIOLOGY | 2009年 / 22卷 / 03期
关键词：
c2-ceramide; Cell viability; Apoptosis; Matrixmetalloproteinases-1; Transforming growth factor-beta; Elastin; GROWTH-FACTOR-BETA; STRATUM-CORNEUM STRUCTURE; HUMAN SKIN; ULTRAVIOLET-RADIATION; GENE-EXPRESSION; ATOPIC-DERMATITIS; DECREASED LEVELS; MESSENGER-RNA; APOPTOSIS; ELASTIN;
D O I：
10.1159/000208168
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
Ceramides are used in skin care and treatment of dermatological diseases. Cell viability and extracellular matrix (ECM) remodeling are important parameters in skin health. Skin photoaging, from exposure to ultraviolet radiation, is associated with epidermal hyperplasia and dermal ECM atrophy caused by alterations in expression of matrixmetalloproteinases (MMPs), elastin and transforming growth factor-beta (TGF-beta). The purpose of this study was to determine the effects of c2-ceramide (ceramide) on the cell viability and expression of TGF-beta, MMP-1 and elastin in cultured keratinocytes and fibroblasts. Ceramide inhibited keratinocyte cell viability by apoptosis and stimulated expression of elastin, MMP-1 and TGF-beta, suggesting improved epidermal functioning by a TGF-beta mechanism. Conversely, ceramide stimulated fibroblast cell growth at the lower concentrations and inhibited the expression of MMP-1, elastin and TGF-beta, which indicates a predominantly beneficial effect in the prevention of photoaging-associated dermal alteration by TGF-beta and non-TGF-beta mechanisms. The regulation of MMP-1 expression by ceramide was transcriptionally mediated and via the activator protein-1 sequence in both keratinocytes and fibroblasts. The study delineates the specific, though differential, beneficial effects of ceramide in the prevention of epidermal hyperplasia and dermal ECM remodeling, associated with photoaging. Copyright (C) 2009 S. Karger AG, Basel
引用
收藏
页码：151 / 157
页数：7
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