Determination of levofloxacin in human urine by adsorptive square-wave anodic stripping voltammetry on a glassy carbon electrode

The adsorption behavior of levofloxacin on a glassy carbon electrode was explored by cyclic and square-wave voltammetry. The drug was accumulated on a glassy carbon electrode and a well-defined oxidation peak was obtained in acetate buffer pH 5.0. Using square-wave anodic stripping voltammetry and accumulation at +0.4 V versus Ag/AgCl (saturated KCl) for 300 s, linear calibration graph was obtained from 6.0 x 10(-9) to 5.0 x 10(-7) M levofloxacin. The detection limit was calculated to be 5.0 x 10(-9) M. The R.S.D. determined from ten determinations at the 1.0 x 10(-7) M level was 1.7%. The method was applied for the direct determination of levofloxacin in diluted urine samples. It was validated using high-performance liquid chromatography (HPLC) as a reference method. (C) 2002 Elsevier Science B.V. All rights reserved.